BLASTN 2.2.24 [Aug-08-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= DPGLEAN11421 (UTR included) (4204 letters) Database: est 9484 sequences; 8,280,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BF01012A1E04 100 4e-20 BF01012A1B09 86 5e-16 BF01056A1B08 76 5e-13 BF01025B2E05 76 5e-13
>BF01012A1E04 Length = 841 Score = 99.6 bits (50), Expect = 4e-20 Identities = 68/74 (91%) Strand = Plus / Plus Query: 157 aaatccgcgtagtgtatccgaaaatatattagtttcatttaaatgaatttaaatgacgaa 216 ||||||||||||| |||||||||||||||||||||||||||||||||| || | |||| Sbjct: 545 aaatccgcgtagtatatccgaaaatatattagtttcatttaaatgaataaaactcgcgaa 604 Query: 217 agtcttagatctca 230 |||||||||||||| Sbjct: 605 agtcttagatctca 618 >BF01012A1B09 Length = 841 Score = 85.7 bits (43), Expect = 5e-16 Identities = 68/75 (90%), Gaps = 1/75 (1%) Strand = Plus / Minus Query: 157 aaatccgcgtagtgtatccgaaaatatattagtttcatttaaatgaatttaaatgacgaa 216 ||||||||||||||||||||||| |||||||||||||||||||||||| || | ||| Sbjct: 778 aaatccgcgtagtgtatccgaaa-tatattagtttcatttaaatgaataaaactcgcgac 720 Query: 217 agtcttagatctcat 231 ||||||||||||||| Sbjct: 719 agtcttagatctcat 705 >BF01056A1B08 Length = 761 Score = 75.8 bits (38), Expect = 5e-13 Identities = 53/58 (91%) Strand = Plus / Minus Query: 174 ccgaaaatatattagtttcatttaaatgaatttaaatgacgaaagtcttagatctcat 231 ||||||||||||||||||||||||||||||| || | ||||||||||||||||||| Sbjct: 153 ccgaaaatatattagtttcatttaaatgaataaaactcgcgaaagtcttagatctcat 96 >BF01025B2E05 Length = 759 Score = 75.8 bits (38), Expect = 5e-13 Identities = 56/62 (90%) Strand = Plus / Plus Query: 171 tatccgaaaatatattagtttcatttaaatgaatttaaatgacgaaagtcttagatctca 230 |||||||||||||| ||||||||||||||||||| || | |||||||||||||||||| Sbjct: 59 tatccgaaaatatactagtttcatttaaatgaataaaactcgcgaaagtcttagatctca 118 Query: 231 tt 232 || Sbjct: 119 tt 120 Database: est Posted date: Jul 14, 2011 2:25 PM Number of letters in database: 8,280,457 Number of sequences in database: 9484 Lambda K H 1.37 0.711 1.31 Gapped Lambda K H 1.37 0.711 1.31 Matrix: blastn matrix:1 -3 Gap Penalties: Existence: 5, Extension: 2 Number of Sequences: 9484 Number of Hits to DB: 415,159 Number of extensions: 7182 Number of successful extensions: 7182 Number of sequences better than 1.0e-10: 4 Number of HSP's gapped: 7177 Number of HSP's successfully gapped: 4 Length of query: 4204 Length of database: 8,280,457 Length adjustment: 18 Effective length of query: 4186 Effective length of database: 8,109,745 Effective search space: 33947392570 Effective search space used: 33947392570 X1: 11 (21.8 bits) X2: 15 (29.7 bits) X3: 50 (99.1 bits) S1: 12 (24.3 bits) S2: 35 (69.9 bits)