>BF01022B1A02
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Migratory profiles

A total of 40 monarch butterflies were used for the microarray analysis. Of the 40, 10 (5 male/5 female) were summer butterflies (designated as SUMMER) and 30 were migrant butterflies. The migrant butterflies were further divided into three groups: 10 (5M/5F) were untreated (FALL); 10 (5M/5F) were treated with methoprene (FALL METHOPRENE), which is a juvenile hormone analog and induces the development of reproductive organs in migrant butterflies; and 10 (5M/5F) were treated with vehicle (control) acetone (FALL VEHICLE).
For potential genes involved in oriented flight behavior, compare the summer group to each of the three fall groups; for the juvenile hormone-response genes, compare the summer and the fall groups, and the methoprene-treated and vehicle-treated migrants.